viogreen labeled cd8a Search Results


cd8 viogreen rea734  (Miltenyi Biotec)
95
Miltenyi Biotec cd8 viogreen rea734
Shown is the frequency of CFSE low CD4 T cells out of total CD4 T cells for all vaccinees, vaccinees per group and for each vaccinee. Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) (peptide pool) and single peptides selected based on epitope mapping of the entire antigen (single peptide). After day 7 of in vitro expansion, cells were stained with antibodies to surface markers (CD3, CD4, and <t>CD8)</t> that enable gating on viable CD4 T cells. CFSE intensity was used to identify and sort CFSE low cells for T cell receptor (TCR) repertoire analysis of antigen-specific CD4 T cells.
Cd8 Viogreen Rea734, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD8+Antibody%2C+anti-human%2C+REAfinity/pmc08824481-5-2-8
Average 95 stars, based on 1 article reviews
cd8 viogreen rea734 - by Bioz Stars, 2026-10
95/100 stars
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apc vio770 anti cd8  (Miltenyi Biotec)
94
Miltenyi Biotec apc vio770 anti cd8
Shown is the frequency of CFSE low CD4 T cells out of total CD4 T cells for all vaccinees, vaccinees per group and for each vaccinee. Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) (peptide pool) and single peptides selected based on epitope mapping of the entire antigen (single peptide). After day 7 of in vitro expansion, cells were stained with antibodies to surface markers (CD3, CD4, and <t>CD8)</t> that enable gating on viable CD4 T cells. CFSE intensity was used to identify and sort CFSE low cells for T cell receptor (TCR) repertoire analysis of antigen-specific CD4 T cells.
Apc Vio770 Anti Cd8, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD8a+Antibody%2C+anti-mouse%2C+REAfinity/pmc09745717-122-16-19
Average 94 stars, based on 1 article reviews
apc vio770 anti cd8 - by Bioz Stars, 2026-10
94/100 stars
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cd8 pe-cy5  (Becton Dickinson)
90
Becton Dickinson cd8 pe-cy5
Shown is the frequency of CFSE low CD4 T cells out of total CD4 T cells for all vaccinees, vaccinees per group and for each vaccinee. Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) (peptide pool) and single peptides selected based on epitope mapping of the entire antigen (single peptide). After day 7 of in vitro expansion, cells were stained with antibodies to surface markers (CD3, CD4, and <t>CD8)</t> that enable gating on viable CD4 T cells. CFSE intensity was used to identify and sort CFSE low cells for T cell receptor (TCR) repertoire analysis of antigen-specific CD4 T cells.
Cd8 Pe Cy5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/anti+cd3/pmc06722213-61-14-16
Average 90 stars, based on 1 article reviews
cd8 pe-cy5 - by Bioz Stars, 2026-10
90/100 stars
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cd19-fitc  (Becton Dickinson)
90
Becton Dickinson cd19-fitc

Cd19 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/anti+cd19/pmc10583083-317-10-19
Average 90 stars, based on 1 article reviews
cd19-fitc - by Bioz Stars, 2026-10
90/100 stars
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cd20-fitc  (Becton Dickinson)
90
Becton Dickinson cd20-fitc

Cd20 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/mabs+antibody/pmc10583083-317-11-19
Average 90 stars, based on 1 article reviews
cd20-fitc - by Bioz Stars, 2026-10
90/100 stars
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cd192 ccr2  (Miltenyi Biotec)
93
Miltenyi Biotec cd192 ccr2

Cd192 Ccr2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD192+(CCR2)+Antibody%2C+anti-mouse%2C+REAfinity/pmc08634681-137-57-59
Average 93 stars, based on 1 article reviews
cd192 ccr2 - by Bioz Stars, 2026-10
93/100 stars
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cd3  (Miltenyi Biotec)
95
Miltenyi Biotec cd3
General gating strategy for identified immune cell subsets and the used marker combination for definition.
Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD3+Antibody%2C+anti-mouse%2C+REAfinity/pmc08634681-137-31-33
Average 95 stars, based on 1 article reviews
cd3 - by Bioz Stars, 2026-10
95/100 stars
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cd11c  (Miltenyi Biotec)
95
Miltenyi Biotec cd11c
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd11c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD11c+Antibody%2C+anti-mouse%2C+REAfinity/pmc08634681-137-21-23
Average 95 stars, based on 1 article reviews
cd11c - by Bioz Stars, 2026-10
95/100 stars
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cd4  (Miltenyi Biotec)
95
Miltenyi Biotec cd4
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD4+Antibody%2C+anti-mouse%2C+REAfinity/pmc08634681-137-46-48
Average 95 stars, based on 1 article reviews
cd4 - by Bioz Stars, 2026-10
95/100 stars
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cd45r b220  (Miltenyi Biotec)
95
Miltenyi Biotec cd45r b220
List containing all antibodies utilized for surface staining of mass cytometry samples.
Cd45r B220, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/CD45R+(B220)+Antibody%2C+anti-mouse/pmc08634681-137-41-43
Average 95 stars, based on 1 article reviews
cd45r b220 - by Bioz Stars, 2026-10
95/100 stars
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ly 6c  (Miltenyi Biotec)
95
Miltenyi Biotec ly 6c
List containing all antibodies utilized for surface staining of mass cytometry samples.
Ly 6c, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/Ly-6C+Antibody%2C+anti-mouse%2C+REAfinity/pmc08634681-137-51-53
Average 95 stars, based on 1 article reviews
ly 6c - by Bioz Stars, 2026-10
95/100 stars
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mouse igg2b percp cy5 5  (SouthernBiotech)
93
SouthernBiotech mouse igg2b percp cy5 5
List containing all antibodies utilized for surface staining of mass cytometry samples.
Mouse Igg2b Percp Cy5 5, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/viogreen+labeled+cd8a/Mouse+Anti-Rat+IgG2b-CY5/pm38799456-162-20-29
Average 93 stars, based on 1 article reviews
mouse igg2b percp cy5 5 - by Bioz Stars, 2026-10
93/100 stars
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Image Search Results


Shown is the frequency of CFSE low CD4 T cells out of total CD4 T cells for all vaccinees, vaccinees per group and for each vaccinee. Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) (peptide pool) and single peptides selected based on epitope mapping of the entire antigen (single peptide). After day 7 of in vitro expansion, cells were stained with antibodies to surface markers (CD3, CD4, and CD8) that enable gating on viable CD4 T cells. CFSE intensity was used to identify and sort CFSE low cells for T cell receptor (TCR) repertoire analysis of antigen-specific CD4 T cells.

Journal: eLife

Article Title: Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination

doi: 10.7554/eLife.68388

Figure Lengend Snippet: Shown is the frequency of CFSE low CD4 T cells out of total CD4 T cells for all vaccinees, vaccinees per group and for each vaccinee. Peripheral blood mononuclear cells from day 60 were labeled with carboxyfluorescein succinimidyl ester (CFSE) and stimulated with a pool of peptides spanning hepatitis B (HB) surface antigen (HBsAg) (peptide pool) and single peptides selected based on epitope mapping of the entire antigen (single peptide). After day 7 of in vitro expansion, cells were stained with antibodies to surface markers (CD3, CD4, and CD8) that enable gating on viable CD4 T cells. CFSE intensity was used to identify and sort CFSE low cells for T cell receptor (TCR) repertoire analysis of antigen-specific CD4 T cells.

Article Snippet: Antibody , CD8-VioGreen (REA734) (recombinant antibodies, REAfinity) , Miltenyi Biotec , Cat# 130-110-684 , FACS (1/50 per test).

Techniques: Labeling, In Vitro, Staining

( A ) Gating strategy started by a lymphocyte gate, followed by gating on viable CD3 + CD8 − T cells. Doublets were excluded using doublet discrimination (area against the height of forward scatter pulse) before gating on CD4 + T cells. Next, CD45RA, CXCR5, CD25, and CD127 were used to identify main subsets of CD4 T cells using Boolean gates as specified in the accompanying table. ( B ) Shown an example of gating for CD154 (CD40L) and CD137 (4-1BB) for cells left unstimulated (left) and cells stimulated with a master peptide pool (right) for an early-converter vaccinee at day 60.

Journal: eLife

Article Title: Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination

doi: 10.7554/eLife.68388

Figure Lengend Snippet: ( A ) Gating strategy started by a lymphocyte gate, followed by gating on viable CD3 + CD8 − T cells. Doublets were excluded using doublet discrimination (area against the height of forward scatter pulse) before gating on CD4 + T cells. Next, CD45RA, CXCR5, CD25, and CD127 were used to identify main subsets of CD4 T cells using Boolean gates as specified in the accompanying table. ( B ) Shown an example of gating for CD154 (CD40L) and CD137 (4-1BB) for cells left unstimulated (left) and cells stimulated with a master peptide pool (right) for an early-converter vaccinee at day 60.

Article Snippet: Antibody , CD8-VioGreen (REA734) (recombinant antibodies, REAfinity) , Miltenyi Biotec , Cat# 130-110-684 , FACS (1/50 per test).

Techniques:

Journal: eLife

Article Title: Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination

doi: 10.7554/eLife.68388

Figure Lengend Snippet:

Article Snippet: Antibody , CD8-VioGreen (REA734) (recombinant antibodies, REAfinity) , Miltenyi Biotec , Cat# 130-110-684 , FACS (1/50 per test).

Techniques: Recombinant, Sequencing, Software, Staining, Virus

Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in different combinations: CD3-FITC (eBioscience); CD19-FITC, CD20-FITC, CD56-FITC, CD25-PE, CCR4-PECy7, CCR6-PerCp/Cy5.5,HLA-ABC-PE, CD3 - BUV395 (BD); CLEC9A-PE, CD1c-PE, CD1c-APC, CD141-PE,CD141-APC, CD278 (ICOS)-VioGreen, CD127 – APC, CD25 -PE and CD45 -VioBright515 (Miltenyi); CD3-FITC, PD-L1-PE and CD45-PECy7 (eBioscience); CD11c-PerCp/Cy5.5, CD4 -BV785, CD8a - BV650, CTLA-4 - BV605, FoxP3 - BV421 and Ki-67 - BV711 (BioLegend); CCR10-PE (R&D Systems); CD14-FITC, CD83-FITC, CD86-FITC, CD40-FITC and HLA-DR-PE (Invitrogen).

Techniques: Purification, Recombinant, Staining, Concentration Assay, Sterility, Multiplex Assay, Labeling, Expressing, Transfection, Software

Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

Figure Lengend Snippet:

Article Snippet: The following antibodies were used in different combinations: CD3-FITC (eBioscience); CD19-FITC, CD20-FITC, CD56-FITC, CD25-PE, CCR4-PECy7, CCR6-PerCp/Cy5.5,HLA-ABC-PE, CD3 - BUV395 (BD); CLEC9A-PE, CD1c-PE, CD1c-APC, CD141-PE,CD141-APC, CD278 (ICOS)-VioGreen, CD127 – APC, CD25 -PE and CD45 -VioBright515 (Miltenyi); CD3-FITC, PD-L1-PE and CD45-PECy7 (eBioscience); CD11c-PerCp/Cy5.5, CD4 -BV785, CD8a - BV650, CTLA-4 - BV605, FoxP3 - BV421 and Ki-67 - BV711 (BioLegend); CCR10-PE (R&D Systems); CD14-FITC, CD83-FITC, CD86-FITC, CD40-FITC and HLA-DR-PE (Invitrogen).

Techniques: Purification, Recombinant, Staining, Concentration Assay, Sterility, Multiplex Assay, Labeling, Expressing, Transfection, Software

General gating strategy for identified immune cell subsets and the used marker combination for definition.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: General gating strategy for identified immune cell subsets and the used marker combination for definition.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Marker

List containing all antibodies utilized for surface staining of mass cytometry samples.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Staining, Mass Cytometry, Marker

General gating strategy for identified immune cell subsets and the used marker combination for definition.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: General gating strategy for identified immune cell subsets and the used marker combination for definition.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Marker

Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Two Tailed Test

List containing all antibodies utilized for surface staining of mass cytometry samples.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Staining, Mass Cytometry, Marker

General gating strategy for identified immune cell subsets and the used marker combination for definition.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: General gating strategy for identified immune cell subsets and the used marker combination for definition.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Marker

Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Influence of diet-induced obesity on circulating murine immune cells. Uniform manifold approximation and projection (UMAP) with clusters from FlowSOM analysis (Ly6G, CD115, CD4, CD11b, CD19, CD3e, TCRgd, Ly6C, NKp46, CD8a, NK1.1, B220, and CD11c) in mice receiving either low-fat diet (LFD) or high-fat diet (HFD) (A) . Z -score normalized heatmap indicating percentages of immune cell populations in lean and obese mice (B) as well as their actual percentages (C) . Statistics: unpaired two-tailed Student’s t -test to compare the populations between lean and obese mice. ▪ indicates p ≤ 0.1, * indicates p ≤ 0.05, ** indicates p ≤ 0.01. Sample sizes: LFD CTRL = 5, HFD CTRL = 5. Data are displayed as mean ± SEM. ns, not significant.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Two Tailed Test

Analysis of essential components of signal transduction of the inflammatory reflex in the spleen. Occurrence of ChAT + CD4 + T cells in the spleen of lean and obese mice (A) . Statistics: Comparison of lean and obese mice was achieved by an unpaired two-tailed Student’s t -test. Comparison of TNF-α + splenic macrophages after LPS stimulation with or without different concentrations of nicotine treatment to the control (B) were analyzed using a two-way ANOVA with repeated measurements followed by an uncorrected Fisher’s LSD test. * indicates p ≤ 0.05. Sample sizes: Each group at for each analysis and condition: n = 5. Data are displayed as mean ± SEM.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: Analysis of essential components of signal transduction of the inflammatory reflex in the spleen. Occurrence of ChAT + CD4 + T cells in the spleen of lean and obese mice (A) . Statistics: Comparison of lean and obese mice was achieved by an unpaired two-tailed Student’s t -test. Comparison of TNF-α + splenic macrophages after LPS stimulation with or without different concentrations of nicotine treatment to the control (B) were analyzed using a two-way ANOVA with repeated measurements followed by an uncorrected Fisher’s LSD test. * indicates p ≤ 0.05. Sample sizes: Each group at for each analysis and condition: n = 5. Data are displayed as mean ± SEM.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Transduction, Comparison, Two Tailed Test, Control

List containing all antibodies utilized for surface staining of mass cytometry samples.

Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: The master mix for blood and spleen samples analyzing immune subsets during the trauma response contained fluorescently labeled antibodies specific to CD11c (VioBlue, Miltenyi Biotec, 130-110-843), CD8a (VioGreen, Miltenyi Biotec, 130-109-330), CD3 (FITC, Miltenyi Biotec, 130-119-798), CD11b (PE, Miltenyi Biotec, 130-113-806), CD45R/B220 (PerCP-Vio700, Miltenyi Biotec, 130-102-218), CD4 (PE-Vio770, Miltenyi Biotec, 130-123-894), Ly-6C (APC-Vio770, Miltenyi Biotec, 130-111-919) and CD192/CCR2 (APC, Miltenyi Biotec, 130-119-658).

Techniques: Staining, Mass Cytometry, Marker